Simultaneous Determination of the Saponification Value, Acid Value, Ester Value, and Iodine Value in Commercially Available Red Fruit Oil (Pandanus conoideus, Lam.) Using 1H qNMR Spectroscopy

Abstract Red fruit oil (RFO) can be extracted from fruits of Pandanus conoideus, Lam., an endogenous plant Papua, Indonesia. It is a commonly used essential original traditional medicine. By applying newly developed quantitative 1 H NMR (qNMR) spectroscopy method for quality assessment, simultaneous determination the saponification value (SV), acid (AV), ester (EV), and iodine (IV) in RFO was possible. Dimethyl sulfone (DMSO 2 ) as internal standard. Optimization parameters, such pulse sequence, relaxation delay time, receiver gain, finally established NMR-based quantification approach. Diagnostic signals standard at δ = 2.98 ppm, SV 2.37–2.20 AV 2.27–2.20 EV 2.37–2.27 IV 5.37–5.27 respectively, were analysis. The validated concerning linearity ( R 0.999), precision (less than 0.83%), repeatability range 99.17–101.17%. Furthermore, this successfully applied to crude RFO, with palmitic oleic addition, nine commercial products. qNMR results respective fat values are accordance methods, seen F - t -test (< 1.65 < 1.66, respectively). fundamental advantages qNMR, its rapidity simplicity, make it feasible existing alternative titration control RFO.